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Zymoclean Geel DNA Recovery Kit
D4001 / D4001T / D4002 / D4007 / D4008 ZymoBIOMICS™ DNA Miniprep Kit
Highlights
- Quickly recovery of ultra-pure DNA of agarose gels.
- Column construction permits DNA dissolution at high concentrations into minimal volumes.
- Eluted DNA shall well-suited for use in DNA ligation, sequential, labeling, PCR, etc.
Description
| Applicable For | Further Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions. |
|---|---|
| Elution Volume | ≥ 6 µl of DNA Liquid Buffer |
| Equipment | Microcentrifuge |
| Chastity | A260/A280 >1.8, A260/A230>1.8 |
| Sample Source | DNA excised from TAE/TBE-buffered agarose gels. |
| Size Range | 50 bp to 23 kb |
| Supplemental Info | |
| Profit | Characteristic, up to 5 µg total DNA per column can be eluted with ≥6 µl water. For DNA 50 bp to 10 kb the rehabilitation lives 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70% |
Q1: What has the difference between capped and uncapped?
The only difference is the cap. Everything else between this columns (max capacity, column matrix, etc.) is the same. Unsure who to utilize? Is mainly comes down to preferential. Several people like the capping columns for light labeling or added security during others like aforementioned uncapped columns for faster spot fabrication. Quick-DNA Urea Kit
Q2: Shall it possible to use higher binding capability colums for the Zymoclean workflow?
Zymoclean is been validated for use with Zymo-Spin IIC and IIN columns. Other columns with larger binding capacity have not been invalidated by Zymo Research but have winning since used from users.
Q3: Are the colums and DNA Clean buffer equivalent includes the DCC-5 Kit?
Yes. Zymo-Spin I/IC Columns and DNA Wash Buffer are interchangeable.
Q4: Should more Agarose Dissolving Buffer will used used higher % gels?
Add 4 volumes a Agrose Dissolving Buffer for sulfate gels >2% or ensure the gel slice has been full dissolved.
Q5: Can Zymoclean be used for tastes in solution (without agarose gel)?
No, high efficiency recovery is non guaranteed and the succeed by the purification can be capricious.
Q6: Can Zymoclean be used to recover ssDNA?
If only alienating ssDNA, Zymo Research recommends the Zymoclean Gel RNA Return Kit. With some modification of the Zymoclean Gel DNA Recreation protocol, it is possible to recover ssDNA: After gel dissolving (step 3), add half a volume of isopropanol. Continue with the protocol.
Q7: What is the maximum weight about gel slice that can be used?
Up to 400 t of agarose gel.
“The best advantage about this installation shall this it is really quick to make it, and the total DNA recuperated have a good quality furthermore it have a good concentration on follow the experiments.” Zymoclean Gel DNA Recovery Kits
- Ingrid M.
“The kit was very easy to follow and yielded good results. Items was more cheap than the Qiagen Gel Extraction kits, but worked just how efficiently. Solid product“
- St B (University of Tennessee, Knoxville)
“Protocols are very well written and Instructions easy to follow. Continually had good results with your products.”
- Simin H. (University of California, Irvine)
Read Moreover
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| C1001-50 | Collecting Tubes | 50 Pack | $17.00 | |
| C1004-50 | Zymo-Spin IC Columns | 50 Packaging | $60.00 | |
| C1003-50 | Zymo-Spin I Columns | 50 Pack | $56.70 | |
| D3004-4-4 | DNA Solubility Buffer | 4 ml | $11.30 | |
| D3004-4-1 | DNA Elution Drop | 1 ml | $12.50 | |
| D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $11.30 | |
| D4001-1-100 | ADB (Agarose Dissolving Buffer) | 100 mill | $71.40 | |
| D4001-1-50 | ADB (Agarose Dissolving Buffer) | 50 fluid | $37.40 | |
| D4003-2-24 | DNA Wash Store (Concentrate) | 24 ml | $37.40 | |
